Last week, our discussions focused on technical choices, particularly in the realm of laboratory equipment and testing methodologies. Members engaged in thoughtful debates about the efficacy of different materials used in chromatography, while also delving into the nuances of dissolution testing outcomes. These conversations highlighted ongoing challenges and innovations within the field, providing practical insights for everyday lab work.
This Week’s Hot Topics
PVDF vs PTFE syringe filters for LC prep
The forum is buzzing about the choice between PVDF and PTFE syringe filters in liquid chromatography preparation. This discussion is crucial for anyone looking to optimize their sample preparation process, as the right filter can significantly impact analytical results. Read more here
Placebo wins dissolution, again
A fascinating thread has emerged around the unexpected performance of placebos in dissolution tests. This raises questions about testing protocols and the variables that might affect results, making it a must-read for those involved in formulation development. Read more here
Stay engaged and keep sharing your valuable experiences. Looking forward to more insightful discussions!
Swapping our phosphate runs from stainless to PEEK plumbing killed the weird tailing on a basic API overnight — “no metal, no mischief.” Just watch max pressure and temp limits, or keep a short stainless stub at the pump if you need rigidity… If you’re stuck with steel, 0.1% TEA in the aqueous can be a decent plan B.
Quick example: we chased odd dissolution swings and found a paddle shaft 2 mm high — re-setting to 25 mm with the setting tool and re-centering the vessels erased a fake ‘placebo > API’. Switching to USP vacuum deaeration tightened Q by about 5%, but if you’re using volatile buffers, helium sparging is safer; @nina_k flagged that, and this FDA note helps: https://www.fda.gov/media/70936/download.